Comparison of protein expression profiles between monolayer and spheroid cell culture of HT-29 cells revealed fragmentation of CK18 in three-dimensional cell culture

A comparative study of the toxicity of two unsubstituted calixarenes consisting of 4 and 6 phenolic fragments, as well as their p-sulfated derivatives, was carried out on the HT-29 colorectal adenocarcinoma cells cultured in two-dimensional (2D) and three-dimensional (3D) formats. It was shown that both unsubstituted calixarenes decrease the viability of tumor cells; calix[4]arene and calix[6]arene exhibited a cytostatic and a cytotoxic effect, respectively. Sulfated derivatives of calixarenes did not have a pronounced toxic effect on HT-29 cells. However, due to their high hydrophilicity and the ability to form adducts with various therapeutic molecules, they can be used for delivery of anticancer drugs. calixarenes, cytotoxicity, HT-29 cells, 2D cell culture, 3D cell culture The work was financially supported by the Russian Science Foundation (project no. 19-15-00397).

Download Full-text

Tumor suppressor gene NGX6 induces changes in protein expression profiles in colon cancer HT-29 cells

Acta Biochimica et Biophysica Sinica ◽

10.1093/abbs/gms042 ◽

2012 ◽

Vol 44 (7) ◽

pp. 584-590 ◽

Cited By ~ 2

Author(s):

Y. Li ◽

Y. Luo ◽

X. Wang ◽

S. Shen ◽

H. Yu ◽

...

Keyword(s):

Colon Cancer ◽

Tumor Suppressor ◽

Protein Expression ◽

Tumor Suppressor Gene ◽

Expression Profiles ◽

Suppressor Gene ◽

Protein Expression Profiles ◽

Ht 29

Download Full-text

Stable Isotope Labeling with Amino Acids in Cell Culture-Based Proteomic Analysis of Ethanol-Induced Protein Expression Profiles in Microglia

Methods in Molecular Biology - Psychiatric Disorders ◽

10.1007/978-1-61779-458-2_35 ◽

2011 ◽

pp. 551-565 ◽

Cited By ~ 9

Author(s):

Bin Liu ◽

David S. Barber ◽

Stanley M. Stevens

Keyword(s):

Amino Acids ◽

Cell Culture ◽

Stable Isotope ◽

Protein Expression ◽

Proteomic Analysis ◽

Isotope Labeling ◽

Expression Profiles ◽

Stable Isotope Labeling ◽

Protein Expression Profiles

Download Full-text

Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

In Vitro Cellular & Developmental Biology - Animal ◽

10.1007/s11626-020-00537-3 ◽

2021 ◽

Author(s):

Terry Riss ◽

O. Joseph Trask

Keyword(s):

Cell Culture ◽

Three Dimensional ◽

3D Culture ◽

Fluorescent Imaging ◽

Culture Models ◽

Assay Methods ◽

Diffusion Rates ◽

Cell Culture Models ◽

Dimensional Cell ◽

Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

Download Full-text